Journal of General Virology

Fig. 1. BMDCs acquire PrP^Sc following in vitro culture with SAF. BMDCs (1x10⁶ cells) were cultured in the absence (BMDC alone) or presence of SAF (equivalent to 10 mg infected brain tissue) for the times indicated. Immunoblots show the accumulation of detergent-insoluble, relatively proteinase K-resistant PrP^Sc within BMDC lysates. Treatment of lysates in the presence (+) or absence (–) of proteinase K (PK) is indicated. SAF (equivalent to 10 mg infected brain tissue) was incubated in medium alone as a control. Following PK treatment, a typical three-band pattern was observed between molecular mass values of 20 and 30 kDa, representing unglycosylated, monoglycosylated and diglycosylated isomers of PrP (in order of increasing molecular mass). SAF equivalent to 50 µg infected brain tissue and/or BMDCs equivalent to 10⁴ cells were loaded per lane.

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